Introduction
R&D Systems now offers a Proteome Profiler™ Human Angiogenesis Antibody Array (Catalog # ARY007). This kit provides a rapid, sensitive, and economical tool to simultaneously detect the relative levels of 55 angiogenesis-related proteins in a single sample. Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules. The use of R&D Systems antibody arrays requires no specialized equipment and eliminates the need to perform multiple immunoprecipitation/Western blot experiments.
- Product Insert
- Protocol for near-infrared (NIR) fluorescence detection
- Troubleshooting Guide
- Assays for analytes represented in the Human Angiogenesis Array Kit
| Simultaneously detect the relative levels of these angiogenesis-related proteins in a single sample. | ||
| Activin A | FGF-7/KGF | PD-ECGF |
| ADAMTS-1 | GDNF | PDGF-AA |
| Angiogenin | GM-CSF | PDGF-AB/PDGF-BB |
| Angiopoietin-1 | HB-EGF | Persephin |
| Angiopoietin-2 | HGF | CXCL4/PF4 |
| Angiostatin/Plasminogen | IGFBP-1 | PlGF |
| Amphiregulin | IGFBP-2 | Prolactin |
| Artemin | IGFBP-3 | Serpin B5/Maspin |
| Tissue Factor/Factor III | IL-1 beta | Serpin E1/PAI-1 |
| CXCL16 | CXCL8/IL-8 | Serpin F1/PEDF |
| DPPIV/CD26 | LAP (TGF-beta 1) | TIMP-1 |
| EGF | Leptin | TIMP-4 |
| EG-VEGF | CCL2/MCP-1 | Thrombospondin-1 |
| Endoglin/CD105 | CCL3/MIP-1 alpha | Thrombospondin-2 |
| Endostatin/Collagen XVIII | MMP-8 | uPA |
| Endothelin-1 | MMP-9 | Vasohibin |
| FGF acidic | NRG1-beta 1 | VEGF |
| FGF basic | Pentraxin 3 | VEGF-C |
| FGF-4 | ||
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma or saliva samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any analyte/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of analyte bound. Chemiluminescence is detected in the same manner as a Western blot.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Sample Data
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| Profiling Angiogenesis-Related Proteins in Human Cancer Tissues. A) The Proteome Profiler Human Angiogenesis Antibody Array (Catalog # ARY007) was used to profile angiogenesis-related proteins in tissues from human prostate, ovarian, and breast cancers. B) Histogram profiles for select analytes were generated by quantifying the mean spot pixel densities from the array membrane using image software. |